Journal: Brain, behavior, and immunity

Article Title: Innately activated TLR4 signal in the nucleus accumbens is sustained by CRF amplification loop and regulates impulsivity

doi: 10.1016/j.bbi.2017.11.008

Figure Lengend Snippet: Percentage of co-staining cells

Article Snippet: Other antibodies were goat anti-GAD67 (glutamic acid decarboxylase 1 (GAD1)) (LifeSpan BioSciences, Seattle, WA, USA, Cat. # LS-B3027-50, RRID: AB_1965223), rabbit anti-CRFR1 (Thermo Fisher Scientific, Waltham, MA, USA, Cat. # 720290, RRID: AB_2633242), rabbit anti-NF-kB p65 (Abcam, Cambridge, MA, USA, Cat. # ab32536, RRID: AB_776751), mouse beta Actin (β-Actin; Proteintech Group, Rosemont, IL, USA, Cat. # 66009-1-Ig, RRID: AB_2687938), Alexa Fluor 488 goat anti-rabbit or donkey anti-goat IgG (H+L; Cat. # {"type":"entrez-nucleotide","attrs":{"text":"A11034","term_id":"489250","term_text":"A11034"}} A11034 , RRID: AB_2576217 or Cat. # {"type":"entrez-nucleotide","attrs":{"text":"A11055","term_id":"490909","term_text":"A11055"}} A11055 , RRID: AB_142672, respectively, Thermo Fisher Scientific), and Alexa Fluor 546 goat anti-mouse or goat anti-rabbit IgG (H+L; Cat.# {"type":"entrez-nucleotide","attrs":{"text":"A11030","term_id":"489248","term_text":"A11030"}} A11030 , RRID: AB_2534089, or Cat. # A11035, RRID: AB_2534093, respectively, Thermo Fisher Scientific).

Techniques: Expressing

Journal: Nature neuroscience

Article Title: An inhibitory hippocampal–thalamic pathway modulates remote memory retrieval

doi: 10.1038/s41593-021-00819-3

Figure Lengend Snippet: Representative images showing immunohistochemical staining in ADn of WT home cage mice with a, inhibitory cell marker GAD67 (repeated in n = 3), or b, excitatory cell marker aCamKII (repeated in n = 3; scale bar: 100 μm).

Article Snippet: Sections were washed with PBS (three times), then incubated with PBS containing goat anti-rabbit ALEXA Fluor 633 (for CTB, 1:500 dilution; Thermo Fisher Scientific Cat. no. A-21070 RRID:AB_2535731) and goat anti-mouse ALEXA Fluor 488 (for GAD67, 1:500 dilution; Thermo Fisher Scientific Cat. no. A-11001 RRID:AB_2534069) secondary antibodies for 24 h at 4 °C.

Techniques: Immunohistochemical staining, Staining, Marker

Journal: Nature neuroscience

Article Title: An inhibitory hippocampal–thalamic pathway modulates remote memory retrieval

doi: 10.1038/s41593-021-00819-3

Figure Lengend Snippet: a,b, WT mice were micro-infused with CTB (n = 3) in the ADn, and retrograde-labeled neurons in CA3 were visualized. Sections were costained with the inhibitory cell marker GAD67. Numerous CTB+ and GAD67+ colocalized cells were visualized in CA3 (white arrows). c,d, To identify inhibitory projections to the ADn, VGAT-Cre mice were micro-infused with AAV-DIO-EYFP in CA3 (n = 3). Strong terminal projections were visualized in the ADn. e–g, To visualize specifically CA3–ADn projections and examine whether they are inhibitory, WT mice were micro-infused with AAV-DIO-mCherry bilaterally in CA3 and AAVrg-Cre-EGFP unilaterally in ADn, and sections were costained with (f) GAD67 (CA3) or (g) VGAT (ADn) (n = 4). Numerous inhibitory CA3–ADn projection neurons in CA3 (mCherry+ and GAD67+, white arrows) and projections in ADn (mCherry+ and VGAT+, white arrows) were visualized (blue scale bars, 100 μm; white scale bars, 50 μm).

Article Snippet: Sections were washed with PBS (three times), then incubated with PBS containing goat anti-rabbit ALEXA Fluor 633 (for CTB, 1:500 dilution; Thermo Fisher Scientific Cat. no. A-21070 RRID:AB_2535731) and goat anti-mouse ALEXA Fluor 488 (for GAD67, 1:500 dilution; Thermo Fisher Scientific Cat. no. A-11001 RRID:AB_2534069) secondary antibodies for 24 h at 4 °C.

Techniques: Labeling, Marker